Journal
/
Genética
/
Lentiviral Mediated Gene Silencing in Human Pseudoislet Prepared in Low Attachment Plates
JoVE Revista
Genética
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Genética
Lentiviral Mediated Gene Silencing in Human Pseudoislet Prepared in Low Attachment Plates

Lentiviral Mediated Gene Silencing in Human Pseudoislet Prepared in Low Attachment Plates

5,277 Views

•

11:24 min

•

May 14, 2019

DOI:

10.3791/59578-v

DOI:
10.3791/59578-v

•

11:24 min
May 14, 2019

•

4 Views
, , , , ,
1Department of Internal Medicine, Carver College of Medicine,University of Iowa, 2Fraternal Order of Eagles Diabetes Research Center,University of Iowa, 3Roy J. Carver Department of Biomedical Engineering,University of Iowa

Summary

Automatically generated

A protocol to create gene modified human pseudoislets from dispersed human islet cells that are transduced by lentivirus carrying short hairpin RNA (shRNA) is presented. This protocol utilizes readily available enzyme and culture vessels, can be performed easily, and produces genetically modified human pseudoislets suitable for functional and morphological studies.

Explore More Videos

LentiviralGene SilencingHuman PseudoisletLow Attachment PlatesIslet FunctionGene Expression ModulationSHR8 TransductionProteolytic EnzymeCollagenolytic EnzymeCell DissociationCMRL MediumFetal Bovine Serum

Read Article

Cite this Article

Liu, S., Harata, M., Promes, J. A., Burand, A. J., Ankrum, J. A., Imai, Y. Lentiviral Mediated Gene Silencing in Human Pseudoislet Prepared in Low Attachment Plates. J. Vis. Exp. (147), e59578, doi:10.3791/59578 (2019).

Download .ris file

Copy

Share Video

.

Copy