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JoVE Journal Genetics
Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation
 

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

Article DOI: 10.3791/59394-v • 09:31 min • April 19th, 2019
April 19th, 2019 •
*1, *2, 2, 1, 3, 3, 1, 2
1Department of Developmental Physiology, Centre for Organismal Studies (COS) Heidelberg, 2Department of Cell Biology, Centre for Organismal Studies (COS) Heidelberg, 3Department of Stem Cell Biology, Centre for Organismal Studies (COS) Heidelberg
* These authors contributed equally

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요약
Automatic Translation

Here, we describe the generation and application of a set of transgenic Arabidopsis thaliana lines enabling inducible, tissue-specific expression in the three main meristems, the shoot apical meristem, the root apical meristem, and the cambium.

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Inducible Cell Type-specific Expression Arabidopsis Thaliana LhGR-mediated Trans-activation Developmental Biology Gene Role Knockout Mutants Constitutive Overexpression Lines Modular Green Gate Cloning System Cyto-specific Expression Meristems Plant Species Transformation Visual Demonstration Trans-activation Effect Stem Meristems Short Apex Meristems Sterilize Seeds Murashige And Skoog Medium PH 5.8 Sucrose Agar Plates Autoclaving Dex Induction Plates Micromolar Concentration Control Plates Root Imaging Stratify Seeds In Darkness And At Four Degrees Celsius Vertical Plate Position Plant Incubator Confocal Laser Scanning Microscopy
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