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Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation
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Genetics
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JoVE Journal Genetics
Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

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9,163 Views

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09:31 min

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April 19, 2019

DOI:

10.3791/59394-v

DOI:
10.3791/59394-v

•

09:31 min
April 19, 2019

•

7 Views
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1Department of Developmental Physiology,Centre for Organismal Studies (COS) Heidelberg, 2Department of Cell Biology,Centre for Organismal Studies (COS) Heidelberg, 3Department of Stem Cell Biology,Centre for Organismal Studies (COS) Heidelberg

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Here, we describe the generation and application of a set of transgenic Arabidopsis thaliana lines enabling inducible, tissue-specific expression in the three main meristems, the shoot apical meristem, the root apical meristem, and the cambium.

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Arabidopsis ThalianaInducible ExpressionCell Type-specificLhGR-mediated Trans-activationGreen Gate CloningMeristemsConfocal MicroscopyDEX InductionSilwet L-77Shoot Apical Meristem

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López-Salmerón, V., Schürholz, A., Li, Z., Schlamp, T., Wenzl, C., Lohmann, J. U., Greb, T., Wolf, S. Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation. J. Vis. Exp. (146), e59394, doi:10.3791/59394 (2019).

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