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Menselijke Colonoid Monolayers studie van interacties tussen ziekteverwekkers, Commensals en Host intestinaal epitheel
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium
DOI:

07:20 min

April 09, 2019

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Chapters

  • 00:04Title
  • 01:22Isolation and Dissociation of Enteroids/Colonoids from 3D Culture
  • 02:39Plating the Enteroid/Colonoid Suspension
  • 04:10Fixation to Preserve and Immunostain Mucus
  • 05:16Results: Establishment and Evaluation of Human Enteroid/Colonoid Monolayers
  • 06:51Conclusion

Summary

자동 번역

Hier presenteren we een protocol bij de menselijke cultuur-enteroid of colonoid monolayers moeten intact barrièrefunctie gastheer epitheliale-microbiota interacties op het cellulaire en biochemisch niveau studeren.

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