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Genetics

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JoVE Journal Genetics
Lentiviral vektor Platform for effektiv levering af Epigenome-redigeringsværktøjer i menneskelige induceret pluripotente stamceller-afledt sygdomsmodeller
 
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Lentiviral vektor Platform for effektiv levering af Epigenome-redigeringsværktøjer i menneskelige induceret pluripotente stamceller-afledt sygdomsmodeller

Article DOI: 10.3791/59241-v • 13:47 min • March 29th, 2019
March 29th, 2019 •
1,2, 3, 3, 1, 1,2, 4, 4, 1,2, 3
1Department of Neurology, Duke University Medical Center, 2Center for Genomic and Computational Biology, Duke University Medical Center, 3Viral Vector Core, Duke University Medical Center, 4Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, Duke University Medical Center

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Målrettet DNA epigenome redigering repræsenterer en kraftfuld terapeutisk tilgang. Denne protokol beskriver produktion, oprensning og koncentration af All-in-one lentiviral vektorer husly CRISPR-dCas9-DNMT3A transgen til epigenome-redigering applikationer i menneskelige inducerede pluripotente stamceller (hiPSC)-afledt neuroner.

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Genetik sag 145 lentiviral vektorer DNMT3A CRISPR-dCas9 SNCA hiPSCs methylering pyrosequencing epigenome-redigering Parkinsons sygdom
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