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Expression, Solubilization, and Purification of Eukaryotic Borate Transporters
Chapters
- 00:04Title
- 00:28Borate Transporter Overexpression in S. cerevisiae
- 01:55Yeast Membrane Harvest
- 03:51Protein Solubilization and Purification
- 05:56Glutaraldehyde Cross-Linking Assay
- 07:09Results: Representative Borate Transporter Purification and Analysis
- 08:18Conclusion
Here we present a protocol to express, solubilize, and purify several eukaryotic borate transporters with homology to the SLC4 transporter family using yeast. We also describe a chemical cross-linking assay to assess the purified homomeric proteins for multimeric assembly. These protocols can be adapted for other challenging membrane proteins.
