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Expression, Solubilization, and Purification of Eukaryotic Borate Transporters
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Expression, Solubilization, and Purification of Eukaryotic Borate Transporters
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March 07, 2019
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Here we present a protocol to express, solubilize, and purify several eukaryotic borate transporters with homology to the SLC4 transporter family using yeast. We also describe a chemical cross-linking assay to assess the purified homomeric proteins for multimeric assembly. These protocols can be adapted for other challenging membrane proteins.
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Flores, S., Feld, A. P., Thurtle-Schmidt, B. H. Expression, Solubilization, and Purification of Eukaryotic Borate Transporters. J. Vis. Exp. (145), e59166, doi:10.3791/59166 (2019).
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