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Detached Leaf Assays to Simplify Gene Expression Studies in Potato During Infestation by Chewing Insect Manduca sexta
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Detached Leaf Assays to Simplify Gene Expression Studies in Potato During Infestation by Chewing Insect Manduca sexta

Detached Leaf Assays to Simplify Gene Expression Studies in Potato During Infestation by Chewing Insect Manduca sexta

6,511 Views

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05:56 min

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May 15, 2019

DOI:

10.3791/59153-v

DOI:
10.3791/59153-v

•

05:56 min
May 15, 2019

•

7 Views
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1Invasive Insect Biocontrol and Behavior Lab,United States Department of Agriculture (USDA), Agricultural Research Service (ARS), 2Genetic Improvement for Fruits and Vegetables Lab,USDA-ARS

Summary

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The presented method creates natural herbivore damaged plant tissue through the application of Manduca sexta larvae to detached leaves of potato. The plant tissue is assayed for expression of six transcription factor homologs involved in early responses to insect herbivory.

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Detached Leaf AssayGene ExpressionPotatoManduca SextaInsect HerbivoryChewing InsectNodal PropagationLarval StagePlant-insect InteractionReproducible DataPlant Genetic Improvement

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Cite this Article

Novak, N. G., Perez, F. G., Jones, R. W., Lawrence, S. D. Detached Leaf Assays to Simplify Gene Expression Studies in Potato During Infestation by Chewing Insect Manduca sexta. J. Vis. Exp. (147), e59153, doi:10.3791/59153 (2019).

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