Journal
/
环境科学
/
Detached Leaf Assays to Simplify Gene Expression Studies in Potato During Infestation by Chewing Insect Manduca sexta
JoVE 杂志
环境科学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 环境科学
Detached Leaf Assays to Simplify Gene Expression Studies in Potato During Infestation by Chewing Insect Manduca sexta

Detached Leaf Assays to Simplify Gene Expression Studies in Potato During Infestation by Chewing Insect Manduca sexta

6,511 Views

•

05:56 min

•

May 15, 2019

DOI:

10.3791/59153-v

DOI:
10.3791/59153-v

•

05:56 min
May 15, 2019

•

7 Views
, , ,
1Invasive Insect Biocontrol and Behavior Lab,United States Department of Agriculture (USDA), Agricultural Research Service (ARS), 2Genetic Improvement for Fruits and Vegetables Lab,USDA-ARS

Summary

Automatically generated

The presented method creates natural herbivore damaged plant tissue through the application of Manduca sexta larvae to detached leaves of potato. The plant tissue is assayed for expression of six transcription factor homologs involved in early responses to insect herbivory.

Explore More Videos

Detached Leaf AssayGene ExpressionPotatoManduca SextaInsect HerbivoryChewing InsectNodal PropagationLarval StagePlant-insect InteractionReproducible DataPlant Genetic Improvement

Read Article

Cite this Article

Novak, N. G., Perez, F. G., Jones, R. W., Lawrence, S. D. Detached Leaf Assays to Simplify Gene Expression Studies in Potato During Infestation by Chewing Insect Manduca sexta. J. Vis. Exp. (147), e59153, doi:10.3791/59153 (2019).

Download .ris file

Copy

Share Video

.

Copy