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A High-throughput Assay to Assess and Quantify Neutrophil Extracellular Trap Formation
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A High-throughput Assay to Assess and Quantify Neutrophil Extracellular Trap Formation
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January 29, 2019
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This protocol describes a highly sensitive and high throughput neutrophil extracellular trap (NET) assay for the semi-automated quantification of ex vivo NET formation by immunofluorescence three-dimensional confocal microscopy. This protocol can be used to evaluate NET formation and degradation after different stimuli and can be used to study potential NET-targeted therapies.
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Arends, E. J., van Dam, L. S., Kraaij, T., Kamerling, S. W., Rabelink, T. J., van Kooten, C., Teng, Y. O. A High-throughput Assay to Assess and Quantify Neutrophil Extracellular Trap Formation. J. Vis. Exp. (143), e59150, doi:10.3791/59150 (2019).
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