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A High-throughput Assay to Assess and Quantify Neutrophil Extracellular Trap Formation
JoVE Journal
免疫学と感染
This content is Free Access.
JoVE Journal 免疫学と感染
A High-throughput Assay to Assess and Quantify Neutrophil Extracellular Trap Formation

A High-throughput Assay to Assess and Quantify Neutrophil Extracellular Trap Formation

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09:59 min

January 29, 2019

DOI:

09:59 min
January 29, 2019

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概要

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This protocol describes a highly sensitive and high throughput neutrophil extracellular trap (NET) assay for the semi-automated quantification of ex vivo NET formation by immunofluorescence three-dimensional confocal microscopy. This protocol can be used to evaluate NET formation and degradation after different stimuli and can be used to study potential NET-targeted therapies.

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