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Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation
This content is Free Access.
1Department of Microbiology and Immunology, Yong Loo Lin School of Medicine,National University of Singapore, 2Immunology Programme, Life Sciences Institute,National University of Singapore, 3Emerging Infectious Diseases Program,Duke-NUS Graduate Medical School, 4Singapore Immunology Network,A*STAR, 5NUS Graduate School for Integrative Sciences and Engineering (NGS),National University of Singapore, 6Department of Immunology, Wright-Fleming Institute,Imperial College London
Chapters
- 00:04Title
- 01:19Plating CHO Cells as Antigen Presenting Cells
- 04:03T Cell Activation Assay
- 07:52CHO Cell MHC Class I Staining
- 09:06Results: Investigation of Co-agonism in Human CD8+ T Cell Activation
- 10:46Conclusion
This protocol describes the use of single chain MHC class I complexes to investigate molecular interactions in human CD8+ T cell activation: generation of engineered antigen presenting cells expressing single chain constructs, culture of human CD8+ T cell clone and T cell activation experiments.
