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Immunology and Infection

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JoVE Journal Immunology and Infection
Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation
 

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation

Article DOI: 10.3791/59126-v • 12:09 min • February 28th, 2019
February 28th, 2019 •
1, 1, 1, 1,2, 3, 4, 3, 1,2,5, 6, 1, 1,2,5
1Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, 2Immunology Programme, Life Sciences Institute, National University of Singapore, 3Emerging Infectious Diseases Program, Duke-NUS Graduate Medical School, 4Singapore Immunology Network, A*STAR, 5NUS Graduate School for Integrative Sciences and Engineering (NGS), National University of Singapore, 6Department of Immunology, Wright-Fleming Institute, Imperial College London

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This protocol describes the use of single chain MHC class I complexes to investigate molecular interactions in human CD8+ T cell activation: generation of engineered antigen presenting cells expressing single chain constructs, culture of human CD8+ T cell clone and T cell activation experiments.

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Keywords: Single Chain MHC Co-agonism Human CD8+ T Cell Activation Nonstimulatory Peptide MHC Agonist Peptide MHC Hepatitis B Virus HIV Gag Tetracycline-inducible Expression CHO Cells T Cell Activation Experiment Trypsinization
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