Journal
/
생체공학
/
Intra-cardiac Side-Firing Light Catheter for Monitoring Cellular Metabolism using Transmural Absorbance Spectroscopy of Perfused Mammalian Hearts
JoVE 신문
생체공학
This content is Free Access.
JoVE 신문 생체공학
Intra-cardiac Side-Firing Light Catheter for Monitoring Cellular Metabolism using Transmural Absorbance Spectroscopy of Perfused Mammalian Hearts

Intra-cardiac Side-Firing Light Catheter for Monitoring Cellular Metabolism using Transmural Absorbance Spectroscopy of Perfused Mammalian Hearts

6,607 Views

•

08:51 min

•

May 12, 2019

DOI:

10.3791/58992-v

DOI:
10.3791/58992-v

•

08:51 min
May 12, 2019

•

3 Views
, ,
1Laboratory of Cardiac Energetics, National Heart, Lung, and Blood Institute,National Institutes of Health, 2Department of Biomedical Engineering,The George Washington University

Summary

Automatically generated

Here we introduce a method for using an intra-ventricle optical catheter in perfused hearts to perform absorbance spectroscopy across the heart wall. The data obtained provides robust information on tissue oxygen tension as well as substrate utilization and membrane potential simultaneously with cardiac performance measures in this ubiquitous preparation.

Explore More Videos

Intra-cardiacSide-firingLight CatheterTransmural Absorbance SpectroscopyPerfused Mammalian HeartsMitochondrial MetabolismMyocardial FunctionFiber Optic CatheterMitral ValveLeft Ventricular Free WallRapid Scanning SpectrometerRoom Light ContaminationData AcquisitionChromophore Reference Spectra

Read Article

Cite this Article

Femnou, A. N., Giles, A., Balaban, R. S. Intra-cardiac Side-Firing Light Catheter for Monitoring Cellular Metabolism using Transmural Absorbance Spectroscopy of Perfused Mammalian Hearts. J. Vis. Exp. (147), e58992, doi:10.3791/58992 (2019).

Download .ris file

Copy

Share Video

.

Copy