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Intra-cardiac Side-Firing Light Catheter for Monitoring Cellular Metabolism using Transmural Absorbance Spectroscopy of Perfused Mammalian Hearts
JoVE Journal
Biotechnik
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JoVE Journal Biotechnik
Intra-cardiac Side-Firing Light Catheter for Monitoring Cellular Metabolism using Transmural Absorbance Spectroscopy of Perfused Mammalian Hearts

Intra-cardiac Side-Firing Light Catheter for Monitoring Cellular Metabolism using Transmural Absorbance Spectroscopy of Perfused Mammalian Hearts

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08:51 min

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May 12, 2019

DOI:

10.3791/58992-v

DOI:
10.3791/58992-v

•

08:51 min
May 12, 2019

•

3 Views
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1Laboratory of Cardiac Energetics, National Heart, Lung, and Blood Institute,National Institutes of Health, 2Department of Biomedical Engineering,The George Washington University

Summary

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Here we introduce a method for using an intra-ventricle optical catheter in perfused hearts to perform absorbance spectroscopy across the heart wall. The data obtained provides robust information on tissue oxygen tension as well as substrate utilization and membrane potential simultaneously with cardiac performance measures in this ubiquitous preparation.

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Intra-cardiacSide-firingLight CatheterTransmural Absorbance SpectroscopyPerfused Mammalian HeartsMitochondrial MetabolismMyocardial FunctionFiber Optic CatheterMitral ValveLeft Ventricular Free WallRapid Scanning SpectrometerRoom Light ContaminationData AcquisitionChromophore Reference Spectra

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Femnou, A. N., Giles, A., Balaban, R. S. Intra-cardiac Side-Firing Light Catheter for Monitoring Cellular Metabolism using Transmural Absorbance Spectroscopy of Perfused Mammalian Hearts. J. Vis. Exp. (147), e58992, doi:10.3791/58992 (2019).

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