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Inducción de Intestinal injerto - versus - host Disease y su evaluación Mini-endoscópica en ratones vivos
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JoVE 신문 면역학 및 감염병학
Induction of Intestinal Graft-versus-host Disease and Its Mini-endoscopic Assessment in Live Mice
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Inducción de Intestinal injerto - versus - host Disease y su evaluación Mini-endoscópica en ratones vivos

9,376 Views

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09:50 min

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February 11, 2019

DOI:

10.3791/58871-v

DOI:
10.3791/58871-v

•

09:50 min
February 11, 2019

•

17 Views
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1Department of Internal Medicine 1, University Erlangen-Nürnberg,University Hospital Erlangen, 2Institute of Pathology, Department of Nephropathology,University Hospital Erlangen, 3Deutsches Zentrum Immuntherapie (DZI),University Hospital Erlangen

Summary

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Aquí, presentamos un protocolo que describe el trasplante alogénico de células madre hematopoyéticas y permite evaluaciones repetitivas mini-endoscópica del colon distal in situ para la presencia, características y severidad de inflamación colónica en vivo ratones de intestinal injerto - versus - host disease.

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Buchele, V., Büttner-Herold, M., Vogler, T., Neurath, M. F., Hildner, K. Induction of Intestinal Graft-versus-host Disease and Its Mini-endoscopic Assessment in Live Mice. J. Vis. Exp. (144), e58871, doi:10.3791/58871 (2019).

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