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Induction of Intestinal Graft-versus-host Disease and Its Mini-endoscopic Assessment in Live Mice
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Immunology and Infection
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JoVE Journal Immunology and Infection
Induction of Intestinal Graft-versus-host Disease and Its Mini-endoscopic Assessment in Live Mice

Induction of Intestinal Graft-versus-host Disease and Its Mini-endoscopic Assessment in Live Mice

English

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9,376 Views

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09:50 min

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February 11, 2019

DOI:

10.3791/58871-v

DOI:
10.3791/58871-v

•

09:50 min
February 11, 2019

•

17 Views
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1Department of Internal Medicine 1, University Erlangen-Nürnberg,University Hospital Erlangen, 2Institute of Pathology, Department of Nephropathology,University Hospital Erlangen, 3Deutsches Zentrum Immuntherapie (DZI),University Hospital Erlangen

Summary

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Here, we present a protocol that describes allogeneic hematopoietic stem cell transplantation and allows repetitive mini-endoscopic evaluations of the distal colon in situ for the presence, characteristics, and severity of colonic inflammation within live mice suffering from intestinal graft-versus-host disease.

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Buchele, V., Büttner-Herold, M., Vogler, T., Neurath, M. F., Hildner, K. Induction of Intestinal Graft-versus-host Disease and Its Mini-endoscopic Assessment in Live Mice. J. Vis. Exp. (144), e58871, doi:10.3791/58871 (2019).

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