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小鼠边缘区 b 细胞剪切流诱导迁移的体外分析
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Analysis of Shear Flow-induced Migration of Murine Marginal Zone B Cells In Vitro
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小鼠边缘区 b 细胞剪切流诱导迁移的体外分析

DOI:
10.3791/58759-v

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08:31 min

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November 26, 2018

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, , , ,
1Institute of Biochemistry and Cell Biology,OVGU University of Magdeburg

Chapters

  • 00:04Title
  • 00:43Preparing the 6-chambered Slides for the Flow Experiment
  • 01:49Fluidics Unit Preparation
  • 03:07Cell Loading
  • 04:04Attaching the Fluidics Unit and Imaging the Cells
  • 05:28Migration Track Analysis
  • 07:02Results: Quantifying Marginal Zone B-cell Shuttling
  • 08:00Conclusion

Summary

자동 번역

자동 번역

边缘区 b 细胞 (mzb) 通过调整其迁移路径的方向来响应剪切流的力。该协议演示了如何使用流体单元、泵、显微镜成像系统和自由软件记录和分析迁移。

Tags

Shear FlowMarginal Zone B CellsMigration AssayICAM-1LeukocytesCytoskeletonIntegrin LigandsChemokinesActivated T CellsFluid PumpMigration BufferShear Stress

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