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淋巴瘤组织学标本中多路荧光组织化学染色、成像与分析
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JoVE 신문 면역학 및 감염병학
Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma
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淋巴瘤组织学标本中多路荧光组织化学染色、成像与分析

19,199 Views

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07:52 min

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January 09, 2019

DOI:

10.3791/58711-v

DOI:
10.3791/58711-v

•

07:52 min
January 09, 2019

•

11 Views
, , , , , , , , ,
1Department of Laboratory Medicine,AnSteel Group General Hospital, 2Cancer Science Institute of Singapore,National University of Singapore, 3Department of Pathology, Yong Loo Lin School of Medicine,National University of Singapore, 4Department of Haematology-Oncology,National University Health System

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Multiplexed Fluorescent ImmunohistochemistryLymphomaTumor MicroenvironmentAntigen Co-expressionSpatial RelationshipAntigen RetrievalMicrowave StrippingPeroxidase BlockTyramide-based Fluorescent ReagentSpectral Imaging

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Cite this Article

Hong, G., Fan, S., Phyu, T., Maheshwari, P., Hoppe, M. M., Phuong, H. M., de Mel, S., Poon, M., Ng, S., Jeyasekharan, A. D. Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma. J. Vis. Exp. (143), e58711, doi:10.3791/58711 (2019).

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