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Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma

Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma

19,199 Views

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07:52 min

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January 09, 2019

DOI:

10.3791/58711-v

DOI:
10.3791/58711-v

•

07:52 min
January 09, 2019

•

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1Department of Laboratory Medicine,AnSteel Group General Hospital, 2Cancer Science Institute of Singapore,National University of Singapore, 3Department of Pathology, Yong Loo Lin School of Medicine,National University of Singapore, 4Department of Haematology-Oncology,National University Health System

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Multiplexed Fluorescent ImmunohistochemistryLymphomaTumor MicroenvironmentAntigen Co-expressionSpatial RelationshipAntigen RetrievalMicrowave StrippingPeroxidase BlockTyramide-based Fluorescent ReagentSpectral Imaging

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Hong, G., Fan, S., Phyu, T., Maheshwari, P., Hoppe, M. M., Phuong, H. M., de Mel, S., Poon, M., Ng, S., Jeyasekharan, A. D. Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma. J. Vis. Exp. (143), e58711, doi:10.3791/58711 (2019).

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