/
/
Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma
Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma
19,295 Views
•
07:52 min
•
January 09, 2019
DOI:
1Department of Laboratory Medicine,AnSteel Group General Hospital, 2Cancer Science Institute of Singapore,National University of Singapore, 3Department of Pathology, Yong Loo Lin School of Medicine,National University of Singapore, 4Department of Haematology-Oncology,National University Health System
Özet
Automatically generated
Here we describe a protocol for multiplex fluorescent immunohistochemical staining and imaging for the simultaneous localization of multiple cancer-associated antigens in lymphoma. This protocol can be extended to the colocalization analysis of biomarkers within all tissue sections.
Read Article
Cite this Article
Hong, G., Fan, S., Phyu, T., Maheshwari, P., Hoppe, M. M., Phuong, H. M., de Mel, S., Poon, M., Ng, S., Jeyasekharan, A. D. Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma. J. Vis. Exp. (143), e58711, doi:10.3791/58711 (2019).
Copy