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Microfluidic Assay for the Assessment of Leukocyte Adhesion to Human Induced Pluripotent Stem Cell-derived Endothelial Cells (hiPSC-ECs)
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Microfluidic Assay for the Assessment of Leukocyte Adhesion to Human Induced Pluripotent Stem Cell-derived Endothelial Cells (hiPSC-ECs)

Microfluidic Assay for the Assessment of Leukocyte Adhesion to Human Induced Pluripotent Stem Cell-derived Endothelial Cells (hiPSC-ECs)

8,424 Views

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06:47 min

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November 26, 2018

DOI:

10.3791/58678-v

DOI:
10.3791/58678-v

•

06:47 min
November 26, 2018

•

7 Views
, , ,
1Department of Anatomy and Embryology,Leiden University Medical Center, 2Department of Applied Stem Cell Technologies,University of Twente

Summary

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This step-by-step protocol provides a detailed description of the experimental setup and data analysis for the assessment of inflammatory responses in hiPSC-ECs and the analysis of leukocyte adhesion under physiological flow.

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Halaidych, O. V., van den Hil, F., Mummery, C. L., Orlova, V. V. Microfluidic Assay for the Assessment of Leukocyte Adhesion to Human Induced Pluripotent Stem Cell-derived Endothelial Cells (hiPSC-ECs). J. Vis. Exp. (141), e58678, doi:10.3791/58678 (2018).

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