This step-by-step protocol provides a detailed description of the experimental setup and data analysis for the assessment of inflammatory responses in hiPSC-ECs and the analysis of leukocyte adhesion under physiological flow.
Halaidych, O. V., van den Hil, F., Mummery, C. L., Orlova, V. V. Microfluidic Assay for the Assessment of Leukocyte Adhesion to Human Induced Pluripotent Stem Cell-derived Endothelial Cells (hiPSC-ECs). J. Vis. Exp. (141), e58678, doi:10.3791/58678 (2018).