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通过 iscat 显微镜对活细胞分泌的单一蛋白质进行无标签成像
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10:55 min
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November 20, 2018
DOI:
1Max Planck Institute for the Science of Light (MPL), 2Area of Scientific Learning,Milligan College, 3Department of Physics,Friedrich Alexander University Erlangen-Nuremberg, 4Department of Internal Medicine 5, Hematology and Oncology,Friedrich Alexander University Erlangen-Nuremberg (FAU), University Hospital Erlangen
Summary
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我们提出了一种实时光学检测单个未标记蛋白质的协议, 因为它们是从活细胞分泌的。这是基于干涉散射 (iscat) 显微镜, 可应用于各种不同的生物系统和配置。
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Cite this Article
Gemeinhardt, A., McDonald, M. P., König, K., Aigner, M., Mackensen, A., Sandoghdar, V. Label-Free Imaging of Single Proteins Secreted from Living Cells via iSCAT Microscopy. J. Vis. Exp. (141), e58486, doi:10.3791/58486 (2018).
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