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Label-Free Imaging of Single Proteins Secreted from Living Cells via iSCAT Microscopy
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JoVE Journal Mühendislik
Label-Free Imaging of Single Proteins Secreted from Living Cells via iSCAT Microscopy

Label-Free Imaging of Single Proteins Secreted from Living Cells via iSCAT Microscopy

DOI:
10.3791/58486-v

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10:55 min

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November 20, 2018

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, , , , ,
1Max Planck Institute for the Science of Light (MPL), 2Area of Scientific Learning,Milligan College, 3'Fizik Bölümü',Friedrich Alexander University Erlangen-Nuremberg, 4Department of Internal Medicine 5, Hematology and Oncology,Friedrich Alexander University Erlangen-Nuremberg (FAU), University Hospital Erlangen

'Bölümler'

  • 00:04'Başlık'
  • 01:18Building the Interferometric Scattering (iSCAT) Microscope
  • 06:48iSCAT Microscopy of Secreting Cells
  • 08:53Results: Label-Free Detection of Proteins Secreted by a Single Cell
  • 09:36Conclusion

Özet

'Otomatik Çeviri'

'Otomatik Çeviri'

We present a protocol for the real-time optical detection of single unlabeled proteins as they are secreted from living cells. This is based on interferometric scattering (iSCAT) microscopy, which can be applied to a variety of different biological systems and configurations.

'Etiketler'

Label-free ImagingSingle ProteinsISCAT MicroscopyBiological NanoparticlesExtinction Cross SectionScattering BackgroundHome-built MicroscopeLUZ CellsMolecular-level ProbingOptical SetupDamped Optical TableHigh Numerical Aperture ObjectiveDiode LaserWide-field LensImmersion OilGlass CoverslipAnti-reflection Coated Beamsplitter

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