/
/
Label-Free Imaging of Single Proteins Secreted from Living Cells via iSCAT Microscopy
This content is Free Access.
Label-Free Imaging of Single Proteins Secreted from Living Cells via iSCAT Microscopy
15,992 Views
•
10:55 min
•
November 20, 2018
DOI:
1Max Planck Institute for the Science of Light (MPL), 2Area of Scientific Learning,Milligan College, 3Department of Physics,Friedrich Alexander University Erlangen-Nuremberg, 4Department of Internal Medicine 5, Hematology and Oncology,Friedrich Alexander University Erlangen-Nuremberg (FAU), University Hospital Erlangen
Summary
Automatically generated
We present a protocol for the real-time optical detection of single unlabeled proteins as they are secreted from living cells. This is based on interferometric scattering (iSCAT) microscopy, which can be applied to a variety of different biological systems and configurations.
Explore More Videos
Label-free ImagingSingle ProteinsISCAT MicroscopyBiological NanoparticlesExtinction Cross SectionScattering BackgroundHome-built MicroscopeLUZ CellsMolecular-level ProbingOptical SetupDamped Optical TableHigh Numerical Aperture ObjectiveDiode LaserWide-field LensImmersion OilGlass CoverslipAnti-reflection Coated Beamsplitter
Read Article
Cite this Article
Gemeinhardt, A., McDonald, M. P., König, K., Aigner, M., Mackensen, A., Sandoghdar, V. Label-Free Imaging of Single Proteins Secreted from Living Cells via iSCAT Microscopy. J. Vis. Exp. (141), e58486, doi:10.3791/58486 (2018).
Copy