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Oral Intubation of Adult Zebrafish: A Model for Evaluating Intestinal Uptake of Bioactive Compounds
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Oral Intubation of Adult Zebrafish: A Model for Evaluating Intestinal Uptake of Bioactive Compounds
Chapters
- 00:04Title
- 00:58Preparing the Equipment for Oral Intubation
- 01:22Zebrafish Anesthetization and Oral Intubation
- 02:40Zebrafish Intestine Dissection
- 03:22Results: Home-made Oral Intubation Device Successfully Delivers Fluorescent Nanoparticles IBsTNFα with Low Average Percent Mortality
- 04:01Conclusion
The protocol describes intubating adult zebrafish with a biologic; then dissecting and preparing the intestine for cytometry, confocal microscopy and qPCR. This method allows administration of bioactive compounds to monitor intestinal uptake and the local immune stimulus evoked. It is relevant for testing the intestinal dynamics of oral prophylactics.
