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成年斑马鱼的口服插管: 一种评价生物活性化合物肠道吸收的模型
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Oral Intubation of Adult Zebrafish: A Model for Evaluating Intestinal Uptake of Bioactive Compounds
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成年斑马鱼的口服插管: 一种评价生物活性化合物肠道吸收的模型

7,633 Views

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04:33 min

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September 27, 2018

DOI:

10.3791/58366-v

DOI:
10.3791/58366-v

•

04:33 min
September 27, 2018

•

6 Views
, ,
1Institute of Biotechnology and Biomedicine (IBB-UAB),Universitat Autònoma de Barcelona, 2Department of Cell Biology, Animal Physiology and Immunology,Universitat Autònoma de Barcelona

Summary

Automatically generated

该协议描述插管成年斑马鱼与生物;然后对肠道进行解剖和制备, 用于细胞术、共焦显微镜和 qPCR。这种方法允许管理生物活性化合物, 以监测肠道摄取和局部免疫刺激诱发。对口腔预防手段的肠道动态检测有重要意义。

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Ji, J., Thwaite, R., Roher, N. Oral Intubation of Adult Zebrafish: A Model for Evaluating Intestinal Uptake of Bioactive Compounds. J. Vis. Exp. (139), e58366, doi:10.3791/58366 (2018).

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