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Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing

Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing

12,486 Views

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12:21 min

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January 07, 2019

DOI:

10.3791/58193-v

DOI:
10.3791/58193-v

•

12:21 min
January 07, 2019

•

5 Views
, , , , , , , ,
1The Pirbright Institute & UK-China Centre of Excellence for Research on Avian Diseases, 2Binzhou Animal Science and Veterinary Medicine Academy & UK-China Centre of Excellence for Research on Avian Diseases

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Herpesvirus of turkeys (HVT) is widely used as a vector platform for the generation of recombinant vaccines against a number of avian diseases. This article describes a simple and rapid approach for the generation of recombinant HVT-vectored vaccines using an integrated NHEJ-CRISPR/Cas9 and Cre-Lox system.

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Tang, N., Zhang, Y., Pedrera, M., Chang, P., Baigent, S., Moffat, K., Shen, Z., Nair, V., Yao, Y. Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing. J. Vis. Exp. (143), e58193, doi:10.3791/58193 (2019).

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