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Detection of Phospholipase C Activity in the Brain Homogenate from the Honeybee
Chapters
- 00:04Title
- 00:39Dissection of the Honeybee Brain
- 02:59Preparation of Brain Homogenates
- 04:21PLC Reaction in Brain Homogenates
- 05:28Detection of Fluorescence Resulting from PLC Activity
- 05:56Results: PLC Activities With and Without PLC Inhibitors in the Mushroom Bodies and Other Brain Regions
- 07:25Conclusion
To test the inhibitory effects of pharmacologic agents on phospholipase C (PLC) in different regions of the honeybee brain, we present a biochemical assay to measure PLC activity in those regions. This assay could be useful for comparing PLC activity among tissues, as well as among bees exhibiting different behaviors.
