To test the inhibitory effects of pharmacologic agents on phospholipase C (PLC) in different regions of the honeybee brain, we present a biochemical assay to measure PLC activity in those regions. This assay could be useful for comparing PLC activity among tissues, as well as among bees exhibiting different behaviors.
Suenami, S., Miyazaki, R., Kubo, T. Detection of Phospholipase C Activity in the Brain Homogenate from the Honeybee. J. Vis. Exp. (139), e58173, doi:10.3791/58173 (2018).