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Quantification of Efferocytosis by Single-cell Fluorescence Microscopy
Chapters
- 00:04Title
- 00:50Quantifying Efferocytic Uptake and Dynamics Using a Fixed Cell Efferocytosis Assay and Inside-out Staining
- 05:14Results: Overnight Culture of Jurkat Cells with Staurosporine Results in Apoptosis of Greater than 95% of Cells
- 05:36Conclusion
Efferocytosis, the phagocytic removal of apoptotic cells, is required to maintain homeostasis and is facilitated by receptors and signaling pathways that allow for the recognition, engulfment, and internalization of apoptotic cells. Herein, we present a fluorescence microscopy protocol for the quantification of efferocytosis and the activity of efferocytic signaling pathways.
