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Quantification of Efferocytosis by Single-cell Fluorescence Microscopy
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Quantification of Efferocytosis by Single-cell Fluorescence Microscopy

Quantification of Efferocytosis by Single-cell Fluorescence Microscopy

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06:15 min

August 18, 2018

DOI:

06:15 min
August 18, 2018

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Summary

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Efferocytosis, the phagocytic removal of apoptotic cells, is required to maintain homeostasis and is facilitated by receptors and signaling pathways that allow for the recognition, engulfment, and internalization of apoptotic cells. Herein, we present a fluorescence microscopy protocol for the quantification of efferocytosis and the activity of efferocytic signaling pathways.

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