Leaf spray mass spectrometry is a direct chemical analysis technique that minimizes the sample preparation and eliminates chromatography, allowing for the rapid detection of small molecules from plant tissues.
Freund, D. M., Sammons, K. A., Makunga, N. P., Cohen, J. D., Hegeman, A. D. Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues. J. Vis. Exp. (136), e57949, doi:10.3791/57949 (2018).