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Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues
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Biochemie
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JoVE Journal Biochemie
Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues

Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues

DOI:
10.3791/57949-v

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06:43 min

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June 21, 2018

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, , , ,
1Department of Horticultural Science, Microbial and Plant Genomics Institute,University of Minnesota, 2Department of Horticultural Science, Department of Plant and Microbial Biology, Microbial and Plant Genomics Institute,University of Minnesota, 3Department of Botany and Zoology,Stellenbosch University

Kapitel

  • 00:05Titel
  • 00:50Modification and Preparation of the MS System for Leaf Spray MS
  • 01:38Preparation of Instrument, Solvents, and Plant Tissue
  • 03:39Data Quality Assessment and Tandem Mass Fragmentation
  • 05:06Results: Detection of Mesembrine Alkaloids from Sceletium tortuosum
  • 06:19Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Leaf spray mass spectrometry is a direct chemical analysis technique that minimizes the sample preparation and eliminates chromatography, allowing for the rapid detection of small molecules from plant tissues.

Tags

Leaf Spray Mass SpectrometryAmbient IonizationPlant MetabolitesSceletium TortuosumMesembrine AlkaloidsPesticidesSample PreparationNano Spray SourceContaminant IonsData File

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