Journal
/
환경과학
/
In Situ Hybridization Techniques for Paraffin-Embedded Adult Coral Samples
JoVE 신문
환경과학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 환경과학
In Situ Hybridization Techniques for Paraffin-Embedded Adult Coral Samples

In Situ Hybridization Techniques for Paraffin-Embedded Adult Coral Samples

DOI:
10.3791/57853-v

•

07:24 min

•

August 31, 2018

•

1Department of Marine Biology and Ecology,University of Miami, Rosenstiel School of Marine and Atmospheric Sciences

Chapters

  • 00:04Title
  • 00:52Removal of Paraffin
  • 01:44Pretreatment of Slides for Preparation of RNA Probe Hybridization
  • 03:03Hybridization of the RNA Probe
  • 04:07Visualization of the RNA Probe
  • 06:18Results: Adult Corals Exposed to Heat Stress Show Spatial Expression of RNA Probes
  • 07:07Conclusion

Summary

자동 번역

자동 번역

The goal of this protocol is to perform in situ hybridization on adult coral samples that have been embedded in paraffin and sectioned onto glass slides. This is a qualitative method used to visualize the spatial expression of an RNA anti-sense probe in paraffin-embedded tissues.

Tags

In Situ HybridizationParaffin-embeddedAdult Coral SamplesCoral Cell BiologyGene ExpressionSpatial Gene ExpressionInvertebrate SystemsDewaxingEthanol GradientProteinase KGlycine PBSSaline Sodium Citrate (SSC)Hybridization BufferProbe DilutionHybridization Oven

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

A Noninvasive Method For In situ Determination of Mating Success in Female American Lobsters (Homarus americanus)

Experimental Protocol for Detecting Cyanobacteria in Liquid and Solid Samples with an Antibody Microarray Chip

A Lipid Extraction and Analysis Method for Characterizing Soil Microbes in Experiments with Many Samples

Preparation of Authigenic Pyrite from Methane-bearing Sediments for In Situ Sulfur Isotope Analysis Using SIMS

Techniques for Investigating the Anatomy of the Ant Visual System

Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA

Bioindication Testing of Stream Environment Suitability for Young Freshwater Pearl Mussels Using In Situ Exposure Methods

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

In Situ Chemotaxis Assay to Examine Microbial Behavior in Aquatic Ecosystems

Determination of Total Lipid and Lipid Classes in Marine Samples

Read Article