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In Situ Hybridization Techniques for Paraffin-Embedded Adult Coral Samples
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In Situ Hybridization Techniques for Paraffin-Embedded Adult Coral Samples

In Situ Hybridization Techniques for Paraffin-Embedded Adult Coral Samples

6,836 Views

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07:24 min

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August 31, 2018

DOI:

10.3791/57853-v

DOI:
10.3791/57853-v

•

07:24 min
August 31, 2018

•

1 Views
1Department of Marine Biology and Ecology,University of Miami, Rosenstiel School of Marine and Atmospheric Sciences

Summary

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The goal of this protocol is to perform in situ hybridization on adult coral samples that have been embedded in paraffin and sectioned onto glass slides. This is a qualitative method used to visualize the spatial expression of an RNA anti-sense probe in paraffin-embedded tissues.

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Cite this Article

Traylor-Knowles, N. In Situ Hybridization Techniques for Paraffin-Embedded Adult Coral Samples. J. Vis. Exp. (138), e57853, doi:10.3791/57853 (2018).

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