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Análisis colorimétrico de lactato intracelular en pequeña escala y del piruvato en el nematodo Caenorhabditis elegans
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Small-Scale Colorimetric Assays of Intracellular Lactate and Pyruvate in the Nematode Caenorhabditis elegans
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Análisis colorimétrico de lactato intracelular en pequeña escala y del piruvato en el nematodo Caenorhabditis elegans

6,775 Views

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09:35 min

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October 15, 2018

DOI:

10.3791/57807-v

DOI:
10.3791/57807-v

•

09:35 min
October 15, 2018

•

16 Views
, ,
1Department of Health Science, School of Sports & Health Science,Daito Bunka University, 2Department of Molecular Life Science, School of Medicine,Tokai University, 3Department of Health Management, School of Health Study,Tokai University

Summary

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Describimos una extracción en pequeña escala modificada y análisis colorimétricos de lactato y piruvato en el nematodo elegans C.. Cuando se utilizan kits de ensayo comerciales, el desarrollo técnico de su sensibilidad y su precisión es importante. Precipitación de proteínas en la extracción es el paso más crítico para la determinación cuantitativa de los metabolitos intracelulares.

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Yanase, S., Yasuda, K., Ishii, N. Small-Scale Colorimetric Assays of Intracellular Lactate and Pyruvate in the Nematode Caenorhabditis elegans. J. Vis. Exp. (140), e57807, doi:10.3791/57807 (2018).

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