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Small-Scale Colorimetric Assays of Intracellular Lactate and Pyruvate in the Nematode Caenorhabditis elegans
Chapters
- 00:04Title
- 00:37Caenorhabditis elegans Synchronized Culture
- 03:21Extraction of Cellular Fraction from C. elegans
- 05:35Lactate, Pyruvate and Protein Assays
- 07:32Results: Efficiency of Lactate and Pyruvate Colorimetric Assays After Protein Precipitation of C. elegans
- 08:26Conclusion
We describe a modified small-scale extraction and colorimetric assays of lactate and pyruvate in the nematode C. elegans. When utilizing commercial assay kits, the technical development of their sensitivity and accuracy is important. Protein precipitation in extraction is the most critical step for the quantitative determination of intracellular metabolites.
