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Biochemistry

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JoVE Journal Biochemistry
Small-Scale Colorimetric Assays of Intracellular Lactate and Pyruvate in the Nematode Caenorhabditis elegans
 

Small-Scale Colorimetric Assays of Intracellular Lactate and Pyruvate in the Nematode Caenorhabditis elegans

Article DOI: 10.3791/57807-v • 09:35 min • October 15th, 2018
October 15th, 2018 •
1,2, 3, 3
1Department of Health Science, School of Sports & Health Science, Daito Bunka University, 2Department of Molecular Life Science, School of Medicine, Tokai University, 3Department of Health Management, School of Health Study, Tokai University

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We describe a modified small-scale extraction and colorimetric assays of lactate and pyruvate in the nematode C. elegans. When utilizing commercial assay kits, the technical development of their sensitivity and accuracy is important. Protein precipitation in extraction is the most critical step for the quantitative determination of intracellular metabolites.

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Small-Scale Colorimetric Assays Intracellular Lactate Pyruvate Nematode Caenorhabditis Elegans Biochem Count Methodology Field Analysis Of Development Rights Protein Precipitation Homogenization E.coli OP50 Strain LB Broth Liquid Medium NGM Plates Nematode Growth Media Agar Plates Sterile Toothpick Agar Adult Stage Stereoscopic Microscope Gravid Hermaphrodites Eggs S-buffer Pasteur Pipette Conical Tube
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