Journal
/
발생학
/
Generation of First Heart Field-like Cardiac Progenitors and Ventricular-like Cardiomyocytes from Human Pluripotent Stem Cells
JoVE 신문
발생학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 발생학
Generation of First Heart Field-like Cardiac Progenitors and Ventricular-like Cardiomyocytes from Human Pluripotent Stem Cells

Generation of First Heart Field-like Cardiac Progenitors and Ventricular-like Cardiomyocytes from Human Pluripotent Stem Cells

6,820 Views

•

08:37 min

•

June 19, 2018

DOI:

10.3791/57688-v

DOI:
10.3791/57688-v

•

08:37 min
June 19, 2018

•

1 Views
, ,
1Sanford Burnham Prebys Medical Discovery Institute, 2Department of Bioengineering,University of California at San Diego

Summary

Automatically generated

Here we describe a scalable method, using a simple combination of Activin A and lentivirus-mediated Id1-overexpression, to generate first heart field-like cardiac progenitors and ventricular-like cardiomyocytes from human pluripotent stem cells.

Explore More Videos

Cardiac ProgenitorsCardiomyocytesHeart FieldPluripotent Stem CellsCell-based TherapyCardiac Disease ModelingId1 LentivirusRHO/ROCK Pathway InhibitorActivin ADifferentiation

Read Article

Cite this Article

Yu, M. S., Spiering, S., Colas, A. R. Generation of First Heart Field-like Cardiac Progenitors and Ventricular-like Cardiomyocytes from Human Pluripotent Stem Cells. J. Vis. Exp. (136), e57688, doi:10.3791/57688 (2018).

Download .ris file

Copy

Share Video

.

Copy