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Generation of First Heart Field-like Cardiac Progenitors and Ventricular-like Cardiomyocytes from Human Pluripotent Stem Cells
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Generation of First Heart Field-like Cardiac Progenitors and Ventricular-like Cardiomyocytes from Human Pluripotent Stem Cells

Generation of First Heart Field-like Cardiac Progenitors and Ventricular-like Cardiomyocytes from Human Pluripotent Stem Cells

6,820 Views

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08:37 min

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June 19, 2018

DOI:

10.3791/57688-v

DOI:
10.3791/57688-v

•

08:37 min
June 19, 2018

•

1 Views
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1Sanford Burnham Prebys Medical Discovery Institute, 2Department of Bioengineering,University of California at San Diego

Summary

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Here we describe a scalable method, using a simple combination of Activin A and lentivirus-mediated Id1-overexpression, to generate first heart field-like cardiac progenitors and ventricular-like cardiomyocytes from human pluripotent stem cells.

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Cardiac ProgenitorsCardiomyocytesHeart FieldPluripotent Stem CellsCell-based TherapyCardiac Disease ModelingId1 LentivirusRHO/ROCK Pathway InhibitorActivin ADifferentiation

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Cite this Article

Yu, M. S., Spiering, S., Colas, A. R. Generation of First Heart Field-like Cardiac Progenitors and Ventricular-like Cardiomyocytes from Human Pluripotent Stem Cells. J. Vis. Exp. (136), e57688, doi:10.3791/57688 (2018).

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