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Geração de insulina tridimensional, livre de andaime expressando Pancreatoids do Mouse progenitoras pancreáticas em Vitro
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09:33 min
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June 02, 2018
DOI:
1Program in Developmental Biology,Baylor College of Medicine, 2Center for Cell and Gene Therapy, Texas Children’s Hospital, and Houston Methodist Hospital,Baylor College of Medicine, 3Molecular and Cellular Biology Department,Baylor College of Medicine, 4Stem Cell and Regenerative Medicine Center,Baylor College of Medicine, 5McNair Medical Institute,Baylor College of Medicine
Summary
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Aqui, apresentamos um protocolo para gerar insulina expressando 3D pancreatoids murino de progenitores pancreático flutuantes e10.5 dissociada e a mesênquima associada.
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Scavuzzo, M. A., Teaw, J., Yang, D., Borowiak, M. Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro. J. Vis. Exp. (136), e57599, doi:10.3791/57599 (2018).
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