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Hele Mount immunofluorescentie en follikel kwantificering van gekweekte muis eierstokken
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Whole Mount Immunofluorescence and Follicle Quantification of Cultured Mouse Ovaries
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Hele Mount immunofluorescentie en follikel kwantificering van gekweekte muis eierstokken

10,406 Views

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08:15 min

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May 02, 2018

DOI:

10.3791/57593-v

DOI:
10.3791/57593-v

•

08:15 min
May 02, 2018

•

9 Views
, ,
1Department of Biomedical Sciences,Cornell University, 2Department of Molecular Biology and Genetics,Cornell University

Summary

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Hier presenteren we een protocol om te kwantificeren follikels in gekweekte eierstokken van jonge Muizen zonder seriële segmenteren. Met behulp van hele orgel immunofluorescentie en weefsel wissen, is fysieke segmenteren vervangen door optische segmenteren. Deze methode voor de bereiding van de monsters en visualisatie handhaaft integriteit van orgel en vergemakkelijkt geautomatiseerde kwantificering van specifieke cellen.

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Cite this Article

Rinaldi, V. D., Bloom, J. C., Schimenti, J. C. Whole Mount Immunofluorescence and Follicle Quantification of Cultured Mouse Ovaries. J. Vis. Exp. (135), e57593, doi:10.3791/57593 (2018).

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