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Developmental Biology

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JoVE Journal Developmental Biology
Whole Mount Immunofluorescence and Follicle Quantification of Cultured Mouse Ovaries
 

Whole Mount Immunofluorescence and Follicle Quantification of Cultured Mouse Ovaries

Article DOI: 10.3791/57593-v • 08:15 min • May 2nd, 2018
May 2nd, 2018 •
1, 2, 1,2
1Department of Biomedical Sciences, Cornell University, 2Department of Molecular Biology and Genetics, Cornell University

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Here, we present a protocol to quantify follicles in cultured ovaries of young mice without serial sectioning. Using whole organ immunofluorescence and tissue clearing, physical sectioning is replaced with optical sectioning. This method of sample preparation and visualization maintains organ integrity and facilitates automated quantification of specific cells.

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Whole Mount Immunofluorescence Follicle Quantification Cultured Mouse Ovaries Automated Follicle Quantification Reproductive Biology Ovarian Fitness Three-dimensional Architecture Embryonic Gonads Young Mice Testes Clean Work Surface Bleach Ethanol Dissecting Microscope Ovary Culture Media Tissue Culture Plates
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