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Whole Mount Immunofluorescence and Follicle Quantification of Cultured Mouse Ovaries
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Whole Mount Immunofluorescence and Follicle Quantification of Cultured Mouse Ovaries
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08:15 min
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May 02, 2018
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Summary
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Here, we present a protocol to quantify follicles in cultured ovaries of young mice without serial sectioning. Using whole organ immunofluorescence and tissue clearing, physical sectioning is replaced with optical sectioning. This method of sample preparation and visualization maintains organ integrity and facilitates automated quantification of specific cells.
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Rinaldi, V. D., Bloom, J. C., Schimenti, J. C. Whole Mount Immunofluorescence and Follicle Quantification of Cultured Mouse Ovaries. J. Vis. Exp. (135), e57593, doi:10.3791/57593 (2018).
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