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培养小鼠卵巢的全贴装免疫荧光和卵泡定量研究
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Whole Mount Immunofluorescence and Follicle Quantification of Cultured Mouse Ovaries
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培养小鼠卵巢的全贴装免疫荧光和卵泡定量研究

10,406 Views

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08:15 min

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May 02, 2018

DOI:

10.3791/57593-v

DOI:
10.3791/57593-v

•

08:15 min
May 02, 2018

•

9 Views
, ,
1Department of Biomedical Sciences,Cornell University, 2Department of Molecular Biology and Genetics,Cornell University

Summary

Automatically generated

在这里, 我们提出一项协议, 以量化卵泡在培养卵巢小鼠没有连续切片。用全器官免疫荧光和组织清除, 用光学切片代替物理切片。这种样品制备和可视化方法保持了器官的完整性, 促进了特定细胞的自动定量。

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Cite this Article

Rinaldi, V. D., Bloom, J. C., Schimenti, J. C. Whole Mount Immunofluorescence and Follicle Quantification of Cultured Mouse Ovaries. J. Vis. Exp. (135), e57593, doi:10.3791/57593 (2018).

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