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Dorsal Root Ganglia Isolation and Primary Culture to Study Neurotransmitter Release
Chapters
- 00:04Title
- 00:32Isolation of Rat Lumbar DRG
- 01:40Primary Culture of Rat DRG
- 04:22Transfection of DRG Cells with NPFFR2 siRNA
- 05:44Release of Neurotransmitters from Primary DRG Cells
- 06:42Results: Stimulation-induced Release of Neurotransmitters from Cultured DRG Cells
- 07:36Conclusion
Dorsal root ganglia (DRG) primary cultures are frequently used to study physiological functions or pathology-related events in sensory neurons. Here, we demonstrate the use of lumbar DRG cultures to detect the release of neurotransmitters after neuropeptide FF receptor type 2 stimulation with a selective agonist.
