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Dorsal Root Ganglia Isolation and Primary Culture to Study Neurotransmitter Release
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JoVE Journal 神経科学
Dorsal Root Ganglia Isolation and Primary Culture to Study Neurotransmitter Release

Dorsal Root Ganglia Isolation and Primary Culture to Study Neurotransmitter Release

46,827 Views

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08:15 min

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October 06, 2018

DOI:

10.3791/57569-v

DOI:
10.3791/57569-v

•

08:15 min
October 06, 2018

•

66 Views
,
1Graduate Institute of Biomedical Sciences, Department of Physiology and Pharmacology,Chang Gung University, 2Healthy Aging Research Center,Chang Gung University, 3Neuroscience Research Center,Chang Gung Memorial Hospital

概要

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Dorsal root ganglia (DRG) primary cultures are frequently used to study physiological functions or pathology-related events in sensory neurons. Here, we demonstrate the use of lumbar DRG cultures to detect the release of neurotransmitters after neuropeptide FF receptor type 2 stimulation with a selective agonist.

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Cite this Article

Lin, Y., Chen, J. Dorsal Root Ganglia Isolation and Primary Culture to Study Neurotransmitter Release. J. Vis. Exp. (140), e57569, doi:10.3791/57569 (2018).

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