Journal
/
면역학 및 감염병학
/
A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo
JoVE 신문
면역학 및 감염병학
Author Produced
This content is Free Access.
JoVE 신문 면역학 및 감염병학
A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo

A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo

DOI:
10.3791/57554-v

•

11:17 min

•

August 11, 2018

•

, , , , , ,
1Department of Infectious Disease Immunology, Center for Vaccine Research,Statens Serum Institut, 2Department of Infectious Diseases,Hvidovre Hospital, 3Institute of Clinical Medicine, Faculty of Health and Medical Sciences,University of Copenhagen, 4Division of Infection and Immunity,University College London

Chapters

  • 00:00Title
  • 01:31Tuberculin Skin Test
  • 03:56Evaluation of Skin Reaction
  • 07:24Harvest of Blister Fluid
  • 08:35Results
  • 10:12Conclusion

Summary

자동 번역

자동 번역

Here, we provide a demonstration of the suction blister cutaneous recall model. The model allows a simple access to study human in vivo adaptive immune responses, for instance in the context of vaccine development.

Tags

Suction BlisterT-cell Recall ResponseAdaptive ImmunityPPDTuberculosisBCG VaccineSkin TestIntradermal InjectionIn VivoAntigen-specific T-cellsCytokines

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

Competitive Homing Assays to Study Gut-tropic T Cell Migration

Induction of Graft-versus-host Disease and In Vivo T Cell Monitoring Using an MHC-matched Murine Model

An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection

The Use of Fluorescent Target Arrays for Assessment of T Cell Responses In vivo

Transplantation of Tail Skin to Study Allogeneic CD4 T Cell Responses in Mice

Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages

Evaluation of Zika Virus-specific T-cell Responses in Immunoprivileged Organs of Infected Ifnar1-/- Mice

Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Read Article